Stumpf, Astrid and Almaca, Joana and Kunzelmann, Karl and Wenners-Epping, Kerstin and Huber, Stephan M. and Haberle, Johannes and Falk, Sabine and Duebbers, Angelika and Walte, Mike and Oberleithner, Hans and Schillers, Hermann (2006) IADS, a decomposition product of DIDS activates a cation conductance in Xenopus oocytes and human erythrocytes: New compound for the diagnosis of cystic fibrosis. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 18 (4-5). pp. 243-252. ISSN 1015-8987,
Full text not available from this repository. (Request a copy)Abstract
DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT ANION CHANNEL; RED-BLOOD-CELLS; CHLORIDE CHANNELS; PLASMA-MEMBRANE; CFTR; EXPRESSION; REGULATOR; LAEVIS; VOLUME; TRANSPORT; CFTR; hydrolysis; hemolysis; gadolinium |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2021 10:01 |
| Last Modified: | 01 Mar 2021 10:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35094 |
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