Heimerl, Susanne and Boettcher, Alfred and Kaul, Harald and Liebisch, Gerhard (2016) Lipid profiling of lipoprotein X: Implications for dyslipidemia in cholestasis. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1861 (8). pp. 681-687. ISSN 1388-1981, 0006-3002
Full text not available from this repository. (Request a copy)Abstract
Lipoprotein X (Lp-X) is an abnormal lipoprotein that may typically be formed in intra- and extrahepatic cholestasis and potentially interfere with lipid analysis in the routine lab. To gain insight into lipid class and species composition, Lp-X, LDL and HDL from cholestatic and control serum samples were subjected to mass spectrometric analysis including phospholipids (PL), sphingolipids, free cholesterol (FC), cholesteryl esters (CE) and bile acids. Our analysis of Lp-X revealed a content of 46% FC, 49% PL with 34% phosphatidylcholine (PC) as main PL component. The lipid species pattern of Lp-X showed remarkable high fractions of mono-unsaturated species including PC 32:1 and PC 34:1 and phosphatidylethanolamine (PE) 32:1 and 34:1. LDL and HDL lipid composition in the same specimens strongly reflected the lipid composition of Lp-X with increased PC 32:1, PC 34:1, PE 32:1, PE 34:1 and FC accompanied by decreased CE compared to controls. Comparison of Lp-X and biliary lipid composition clearly indicates that Lp-X does not originate from a sole release of bile lipids. Moreover, these data present evidence for increased hepatic fatty add and PL synthesis which may represent a reaction to high hepatic FC level observed during cholestasis. (C) 2016 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; LECITHIN-CHOLESTEROL ACYLTRANSFERASE; CHARACTERIZING OBSTRUCTIVE-JAUNDICE; LOW-DENSITY LIPOPROTEINS; COENZYME-A REDUCTASE; LIVER-DISEASE; HOMOGENEOUS ASSAYS; LDL-CHOLESTEROL; ESI-MS/MS; Cholestasis; Lp-X; Lipoproteins; Mass spectrometry; Lipidomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Mar 2019 13:52 |
| Last Modified: | 28 Mar 2019 13:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3513 |
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