Endl, J and Rosinger, S and Schwarz, B and Friedrich, Sven-Olaf and Rothe, G and Karges, W and Schlosser, M and Eiermann, T and Schendel, DJ and Boehm, Bernhard O. (2006) Coexpression of CD25 and OX40 (CD134) receptors delineates autoreactive T-cells in type 1 diabetes. DIABETES, 55 (1). pp. 50-60. ISSN 0012-1797, 1939-327X
Full text not available from this repository. (Request a copy)Abstract
T-cell-mediated loss of pancreatic beta-cells is the crucial event in the development of type 1 diabetes. The phenotypic characteristics of disease-associated T-cells in type I diabetes have not yet been defined. The negative results from two intervention trials (the Diabetes Prevention Trial-Type 1 Diabetes and the European Nicotinamide Diabetes Intervention Trial) illustrate the need for technologies to specifically monitor ongoing autoimmune reactions. We used fluorescence-activated cell sorter analysis to study surface marker expression on T-cell lines specific for two major type 1 diabetes autoantigens, GAD65 and proinsulin. We then applied this knowledge in a cross-sectional approach to delineate the phenotype of circulating memory T-cells. The autoreactive T-cells of patients could be distinguished from those of control subjects by their coexpression of CD25 and CD134. Autoantigen-specific T-cells that recognized multiple GAD65- and preproinsulin-derived peptides and coexpressed CD25(+)CD134(+) were confined to patients (n = 32) and pre-diabetic probands (n = 5). Autoantigen-reactive T-cells in control subjects (n = 21) were CD25(+)CD134(-) and recognized fewer autoantigen-derived peptides. Insulin therapy did not induce CD25(+)CD134(+) T-cells in type 2 diabetic patients. The coexpression of CD25 and the costimulatory molecule CD134 on memory T-cells provides a novel marker for type 1 diabetes-associated T-cell immunity. The CD134 costimulatory molecule may also provide a novel therapeutic target in type 1 diabetes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLUTAMIC-ACID DECARBOXYLASE; IMMUNE-RESPONSE; IDDM PATIENTS; INSULIN; IDENTIFICATION; EPITOPES; AUTOANTIBODIES; INDIVIDUALS; MEMORY; AUTOIMMUNITY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2021 13:44 |
| Last Modified: | 01 Mar 2021 13:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35139 |
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