Windbichler, Michaela and Echtenacher, Bernd and Takahashi, Kazue and Ezekowitz, R. Alan B. and Schwaeble, Wilhelm J. and Jensenius, Jens C. and Maennel, Daniela N. (2006) Investigations on the involvement of the lectin pathway of complement activation in anaphylaxis. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 141 (1). pp. 11-23. ISSN 1018-2438,
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Background: Systemic anaphylaxis is the most severe form of immediate hypersensitivity reaction. The activation of the complement system occurs during anaphylactic shock. The purpose of this study was to determine in a mouse model whether the lectin pathway of complement activation is involved in anaphylaxis. Methods: To see whether the lectin pathway is involved in anaphylactic shock, serum mannan-binding lectin (MBL) levels were measured after passive anaphylaxis. Also MBL expression and binding to potential ligands were investigated. To determine whether complement or mast cell activation is essential for hypothermia in anaphylactic shock, mouse strains deficient in MBL-A and MBL-C, C1q, factors B and C2, C5, C5aR, or mast cells were tested. Results: After antigenic challenge a marked drop in body temperature as well as a rapid decrease in serum MBIL levels were observed. The decrease of serum MBL levels in shock could not be attributed to MBL binding to immune complexes or tissues, but an interaction of MBL with mast cell-derived proteoglycans was seen. In contrast to mast cell-deficient mice, none of the complement-deficient mouse strains were protected from shock-associated hypothermia. Conclusions: These results indicate that neither MBL nor activation of the complement cascade is crucial for the induction of anaphylaxis. In contrast mast cell activation is associated with the development of hypothermia and possibly the observed decrease in serum MBL levels. Copyright (c) 2006 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MANNAN-BINDING LECTIN; MAST-CELLS; DEFICIENT MICE; SYSTEMIC-ANAPHYLAXIS; SERUM COMPLEMENT; EPITHELIAL-CELLS; IMMUNE DEFENSE; FACTOR-B; HEPARIN; IGE; mannan-binding lectin; mast cells; anaphylactic shock; proteoglycans; chondroitin sulphate |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Mar 2021 08:57 |
| Last Modified: | 02 Mar 2021 08:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35179 |
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