Blattner, Christian and Zoumpanioti, Maria and Kroner, Juergen and Schmeer, Georg and Xenakis, Aristotelis and Kunz, Werner (2006) Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide. JOURNAL OF SUPERCRITICAL FLUIDS, 36 (3). pp. 182-193. ISSN 0896-8446, 1872-8162
Full text not available from this repository. (Request a copy)Abstract
Lipases from Candida antarctica and Mucor miehei were encapsulated in lecithin water-in-oil (w/o) microemulsion-based organogels (MBG). These gels were formulated with either hydroxypropylmethyl cellulose (HPMC) or gelatin. The esterification of lauric acid and 1-propanol catalyzed by these MBGs was examined in supercritical carbon dioxide (scCO(2) 35 degrees C, 110 bar) as solvent for the substrates. The results were compared to those obtained with the reference substrate solvent isooctane. It turned out that the initial rates of this model reaction in scCO(2) were higher than those observed in the reference system. Various parameters affecting the biocatalysis such as pressure, alcohol and acid chain length, and gel composition were investigated. Kinetic studies showed that the ester synthesis catalyzed by the immobilized C. antarctica lipase occurs via a Ping Pong Bi Bi mechanism in which only inhibition by excess of alcohol was identified. Values of all kinetic parameters were determined. In addition, experiments on the reusability of these gels in scCO(2) were carried out and the state of water within the organogel was examined with the help of differential scanning calorimetry. The present study shows that biocatalysis using MBGs in scCO(2) is a promising alternative to other bioconversion processes. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-OIL MICROEMULSIONS; CHROMOBACTERIUM-VISCOSUM LIPASE; CATALYZED ESTERIFICATION; IMMOBILIZED LIPASE; ESTER SYNTHESIS; WATER; GELS; GELATIN; ACID; TRANSESTERIFICATION; microemulsion; organogels; lipases; enzymatic reaction; supercritical CO2 |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Mar 2021 06:58 |
| Last Modified: | 03 Mar 2021 06:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35209 |
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