Giesecke, Astrid V. and Fang, Rui and Joung, J. Keith (2006) Synthetic protein-protein interaction domains created by shuffling Cys(2)HiS(2) zinc-fingers. MOLECULAR SYSTEMS BIOLOGY, 2: 2006.0011. ISSN 1744-4292,
Full text not available from this repository. (Request a copy)Abstract
Cys(2)His(2) zinc-fingers (C2H2 ZFs) mediate a wide variety of protein-DNA and protein-protein interactions. DNA-binding C2H2 ZFs can be shuffled to yield artificial proteins with different DNA-binding specificities. Here we demonstrate that shuffling of C2H2 ZFs from transcription factor dimerization zinc-finger (DZF) domains can also yield two-finger DZFs with novel protein-protein interaction specificities. We show that these synthetic protein-protein interaction domains can be used to mediate activation of a single-copy reporter gene in bacterial cells and of an endogenous gene in human cells. In addition, the synthetic two-finger domains we constructed can also be linked together to create more extended, four-finger interfaces. Our results demonstrate that shuffling of C2H2 ZFs can yield artificial protein-interaction components that should be useful for applications in synthetic biology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GCN4 LEUCINE-ZIPPER; DNA-BINDING PROTEINS; TRANSCRIPTION FACTOR; COILED-COIL; IKAROS FAMILY; CRYSTAL-STRUCTURE; RECOGNITION CODE; GENE-EXPRESSION; DRUG DISCOVERY; PHAGE DISPLAY; artificial transcription factor; Cys(2)His(2) zinc-finger; domain shuffling; protein engineering; protein-protein interactions |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Mar 2021 07:38 |
| Last Modified: | 03 Mar 2021 07:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35229 |
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