Schmid, FX and Floerchinger, B and Vudattu, NK and Eissner, G and Haubitz, M and Holler, E and Andreesen, R and Birnbaum, DE (2006) Direct evidence of endothelial injury during cardiopulmonary bypass by demonstration of circulating endothelial cells. PERFUSION-UK, 21 (3). pp. 133-137. ISSN 0267-6591,
Full text not available from this repository. (Request a copy)Abstract
Endothelial activation is considered a key process in the development of a whole body inflammatory response secondary to cardiopulmonary bypass (CPB). Increased levels of a multitude of soluble mediators have been described as being released during and after cardiac surgery. Circulating endothelial cells have recently been established as a novel marker of endothelial damage in a variety of vascular disorders. Blood samples from 20 patients undergoing elective coronary artery bypass surgery were obtained preoperatively and 1, 6, 12, 24, and 48 h after termination of CPB. Control samples were obtained from ten healthy volunteers. Circulating endothelial cells (CEC) were isolated with immunomagnetic anti-CD146-coated Dynabeads, and counted in a Nageotte chamber. Low numbers of CEC were observed in healthy control volunteers (129 +/- 6 cells/mL; median: 9 cells/mL). CEC numbers were already significantly elevated in all patients before CPB, and there was a further significant increase after weaning from CPB (maximum increase at 6 h after CPB: 73 +/- 30 cells/mL; range: 30-153 cells/mL, p < 0.001). The number of CEC provides further and direct evidence that CPB is associated with a pronounced endothelial injury and/or damage. CEC appear to be most useful markers for vascular endothelial activation because they are specific, stable, and circulating components of injured vessel wall.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VON-WILLEBRAND-FACTOR; VASCULAR DISORDERS; HEART-SURGERY; ACTIVATION; DAMAGE; DYSFUNCTION; MARKERS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Mar 2021 08:50 |
| Last Modified: | 03 Mar 2021 08:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35259 |
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