Mueller, Rainer and Abke, Jochen and Schnell, Edith and Macionczyk, F. and Gbureck, U. and Mehrl, R. and Ruszczak, Z. and Kujat, Richard and Englert, Carsten and Nerlich, Michael and Angele, Peter (2005) Surface engineering of stainless steel materials by covalent collagen immobilization to improve implant biocompatibility. BIOMATERIALS, 26 (34). pp. 6962-6972. ISSN 0142-9612, 1878-5905
Full text not available from this repository. (Request a copy)Abstract
It was shown recently that the deposition of thin films of tantalum and tantalum oxide enhanced the long-term biocompatibility of stainless steel biomaterials due to an increase in their corrosion resistance. In this study, we used this tantalum oxide coating as a basis for covalent immobilization of a collagen layer, which should result in a further improvement of implant tissue integration. Because of the high degradation rate of natural collagen in vivo, covalent immobilization as well as carbodiimide induced cross-linking of the protein was performed. It was found that the combination of the silane-coupling agent aminopropyl triethoxysilane and the linker molecule N,N'-disulphosuccinimidyl suberate was a very effective system for collagen immobilizing. Mechanical and enzymatic stability testing revealed a higher stability of covalent bound collagen layers compared to physically adsorbed collagen layers. The biological response induced by the surface modifications was evaluated by in vitro cell culture with human mesenchymal stem cells as well as by in vivo subcutaneous implantation into nude mice. The presence of collagen clearly improved the cytocompatibility of the stainless steel implants which, nevertheless, significantly depended on the cross-linking degree of the collagen layer. (c) 2005 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CROSS-LINKED COLLAGEN; TITANIUM-ALLOY; CELL-ADHESION; AMINO-GROUPS; I COLLAGEN; PROLIFERATION; BIOMATERIALS; ATTACHMENT; DIFFERENTIATION; OSTEOBLASTS; surface modification; steel; tantalum; collagen; coupling agents; cross-linking; mesenchymal stem cells |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > PD Dr. Rainer Müller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Mar 2021 10:32 |
| Last Modified: | 04 Mar 2021 10:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35336 |
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