Muller, Silke and Hoffmann, Petra and Esche, Ulrich von der and Mach, Jean-Pierre and Gorczynski, Reginald M. and Waelli, Thierry and Alexander, Christian and Zahringer, Ulrich and Rietschel, Ernst T. and Bessler, Wolfgang G. and Westphal, Otto (2005) A fetal sheep liver extract containing immunostimulatory substances including LPS acts as leukocyte activator in cells of LPS responder and non responder mice. INTERNATIONAL IMMUNOPHARMACOLOGY, 5 (13-14). pp. 1809-1819. ISSN 1567-5769, 1878-1705
Full text not available from this repository. (Request a copy)Abstract
Purified fractions from a fetal sheep liver extract (FSLE) were investigated, in a murine model, for induction of leukocyte stimulating activities. The fractions FSLE-1 and FSLE-2 induced splenocyte proliferation in vitro in C57B1/10ScSn (LPS responder) mice comparable to LPS, and in C57BL/10ScCr (LPS non responder) mice. They also stimulated the release of nitrogen radicals in bone marrow-derived macrophages (BMDM) from several mouse inbred strains including both C57Bl/ 10ScSn and C57Bl/10ScCr mice. Stimulation of NO production could be blocked by L-NMMA, an inhibitor of iNOS, and enhanced by the simultaneous addition of IFN-gamma. Moreover, stimulation of macrophages by FSLE-1 and FSLE-2 induced a cytostatic effect of the activated macrophages for Abelson 8-1 tumor cells. The stimulatory activity of the purified fractions is partially due to trace amounts of LPS derived from the fetal liver extract which was enriched during purification. Our results may help to explain the beneficial effect of the extract in patients which has been observed clinically. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MARROW-DERIVED MACROPHAGES; TUMOR-NECROSIS-FACTOR; C57BL/10SCCR MICE; NITRIC-OXIDE; CYTO-TOXICITY; L-ARGININE; LIPOPOLYSACCHARIDE; INDUCTION; GENE; UNRESPONSIVENESS; fetal sheep liver extract; nitric oxide; tumor cytostasis; lipopolysaccharide |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Apr 2021 09:02 |
| Last Modified: | 14 Apr 2021 09:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35362 |
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