Bauduin, Pierre and Touraud, Didier and Kunz, Werner and Savelli, Marie-Pierre and Pulvin, Sylviane and Ninham, Barry W. (2005) The influence of structure and composition of a reverse SDS microemulsion on enzymatic activities and electrical conductivities. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 292 (1). pp. 244-254. ISSN 0021-9797, 1095-7103
Full text not available from this repository. (Request a copy)Abstract
The activity of the enzyme horse radish peroxidase (HRP) is studied in a series of reverse microemulsions composed of dodecane, aqueous buffer, sodium dodecylsufate (SDS) and alcohols of the homologous series 1-butanol to 1-octanol. The HRP catalyzed reaction is the oxidation of a classical water soluble substrate, the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) by hydrogen peroxide. In parallel electrical conductivity measurements are performed on the same solutions. The structural changes in the microemulsions, as inferred by the conductivity measurements, correlate remarkably well with the changes in the enzymatic activities. In particular it is found that (a) the maximum activity of the enzyme is always related to its optimum hydration and that this hydration can be related to the microemulsion structures, (b) the enzyme inhibition caused by the alcohols in microemulsions is a consequence of both the solubility of the alcohols in the buffer and the rigidity of the interfacial film. Consequently, it can be concluded that enzymatic activity measurements are a valuable tool to study confined systems such as microemulsions and, in particular, the amount of available hydration water. Enzymatic activities can be finely tuned by small changes in microemulsion structures, probably in a predictive way. (c) 2005 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COSURFACTANT CHAIN-LENGTH; HORSERADISH-PEROXIDASE; HYDROGEN-PEROXIDE; MICRO-EMULSIONS; CATALYTIC-ACTIVITY; ORGANIC-SOLVENTS; KERATIN CYSTINE; ALPHA-CHYMOTRYPSIN; DODECYL-SULFATE; SINGLET OXYGEN; anionic surfactant; reverse microemulsion; enzymatic reaction; alcohols; horse radish peroxidase; conductivity; hydration |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Apr 2021 09:34 |
| Last Modified: | 14 Apr 2021 09:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35372 |
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