Farkas, Laszlo and Hahn, Marie-Christine and Schmoczer, Marc and Jentsch, Nico and Kratzel, Kirsten and Pfeifer, Michael and Schulz, Christian (2005) Expression of CXC chemokine receptors 1 and 2 in human bronchial epithelial cells. CHEST, 128 (5). pp. 3724-3734. ISSN 0012-3692,
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Introduction: CXC chemokine receptor 1 (CXCR1) and CXC chemokine receptor 2 (CXCR2) have been shown to play an important role in transepithelial migration of neutrophil granulocytes during inflammation in various tissues. This study investigated the regulation of gene expression and surface expression of CXCR1 and CXCR2 in a human bronchial epithelial cell line (BEAS-2B), as well as in primary bronchial epithelial cells (PBECs) from 10 COPD patients and 10 control subjects. Methods and results: The transcription expression of CXCR1 and CXCR2 was quantitatively assessed by means of real-time polymerase chain reaction (PCR) under various inflammatory conditions. Flow cytometry was used to measure CXCR1 and CXCR2 surface expression. There was a low baseline expression of CXCR1 and CXCR2 in real-time PCR in PBECs from COPD patients and control subjects as well as in BEAS-2B cells, and no significant regulation occurred under various inflammatory conditions in PBECs and BEAS-2B cells. Furthermore, unstimulated surface expression of CXCR1 and CXCR2 on BEAS-2B cells was very low, and no significant regulation was detectable under time-dependent inflammatory stimulation up to 24 h. Conclusion: Various inflammatory responses that are of potential relevance in COPD pathophysiology do not affect transcription regulation and surface expression of the interleukin-8 receptors CXCR1 and CXCR2 on human bronchial epithelial cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OBSTRUCTIVE PULMONARY-DISEASE; NECROSIS-FACTOR-ALPHA; RECEPTOR EXPRESSION; INTERLEUKIN-8 RECEPTOR; AIRWAY INFLAMMATION; CHEMOKINE RECEPTORS; ADHESION MOLECULES; INDUCED SPUTUM; EXACERBATIONS; ACTIVATION; BEAS-2B; bronchial epithelium; chemokine receptor; CXC chemokine receptor 1; CXC chemokine receptor 2; flow cytometry; real-time amplification |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Apr 2021 09:49 |
| Last Modified: | 23 Apr 2021 09:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35455 |
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