Arndt, S. and Poser, I. and Schubert, T. and Moser, M. and Bosserhoff, Anja Katrin (2005) Cloning and functional characterization of a new Ski homolog, Fussel-18, specifically expressed in neuronal tissues. LABORATORY INVESTIGATION, 85 (11). pp. 1330-1341. ISSN 0023-6837, 1530-0307
Full text not available from this repository. (Request a copy)Abstract
The Sloan Kettering Virus ( Ski) family of nuclear oncoproteins represses transforming growth factor-beta (TGF-beta) signaling through inhibition of transcriptional activity of Smad proteins. Here, we report the discovery of a new functional Smad suppressing element on chromosome 18 (Fussel-18). Fussel-18 encodes for a protein of 297 amino acids sharing characteristic structural features, significant homology and similar genomic organization with the homolog Ski family members, Ski and Ski-related novel sequence (Sno). In contrast to Ski and Sno, which are ubiquitously expressed in human tissues, in situ hybridization, RT-PCR, Western blot and immunohistochemistry revealed a highly specific expression pattern for Fussel-18 in neuronal tissues, especially in the cerebellum, the spinal cord and dorsal root ganglia, during both embryogenesis and adult stage. Functionally, we determined interaction of Fussel-18 with Smad 2 and Smad 3 together with an inhibitory activity on TGF-beta signaling. Fussel-18 is the first example of a Smad-binding protein with a highly restricted expression pattern within the nervous system.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSFORMING-GROWTH-FACTOR; FACTOR-BETA-S; C-SKI; NERVOUS-SYSTEM; MEDIATED REPRESSION; ONCOPROTEIN SKI; SMAD PROTEINS; SNON; ACTS; LOCALIZATION; cerebellum; transforming growth factor; inhibitor; ski family; Smad; signaling |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Apr 2021 12:17 |
| Last Modified: | 23 Apr 2021 12:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35483 |
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