Baldwin, J. G. and Wagner, F. and Martine, L. C. and Holzapfel, B. M. and Theodoropoulos, C. and Bas, O. and Savi, F. M. and Werner, C. and De-Juan-Pardo, E. M. and Hutmacher, D. W. (2017) Periosteum tissue engineering in an orthotopic in vivo platform. BIOMATERIALS, 121. pp. 193-204. ISSN 0142-9612, 1878-5905
Full text not available from this repository. (Request a copy)Abstract
The periosteum plays a critical role in bone homeostasis and regeneration. It contains a vascular component that provides vital blood supply to the cortical bone and an osteogenic niche that acts as a source of bone-forming cells. Periosteal grafts have shown promise in the regeneration of critical size defects, however their limited availability restricts their widespread clinical application. Only a small number of tissue-engineered periosteum constructs (TEPCs) have been reported in the literature. A current challenge in the development of appropriate TEPCs is a lack of pre-clinical models in which they can reliably be evaluated. In this study, we present a novel periosteum tissue engineering concept utilizing a multiphasic scaffold design in combination with different human cell types for periosteal regeneration in an orthotopic in vivo platform. Human endothelial and bone marrow mesenchymal stem cells (BM-MSCs) were used to mirror both the vascular and osteogenic niche respectively. Immunohistochemistry showed that the BM-MSCs maintained their undifferentiated phenotype. The human endothelial cells developed into mature vessels and connected to host vasculature. The addition of an in vitro engineered endothelial network increased vascularization in comparison to cell-free constructs. Altogether, the results showed that the human TEPC (hTEPC) successfully recapitulated the osteogenic and vascular niche of native periosteum, and that the presented orthotopic xenograft model provides a suitable in vivo environment for evaluating scaffold-based tissue engineering concepts exploiting human cells. Crown Copyright (C) 2016 Published by Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; ACELLULAR DERMAL MATRIX; BLOOD-VESSELS; BONE; FRACTURE; RECEPTOR; VASCULATURE; EXPRESSION; SCAFFOLDS; MICE; Periosteum; Tissue engineering & regenerative medicine; Melt electrospinning writing; Star-PEG heparin hydrogel; Mesenchymal stem cells; Human umbilical vein endothelial cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 14 Feb 2019 10:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/355 |
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