Neubauer, Markus and Hacker, Michael and Bauer-Kreisel, Petra and Weiser, Barbara and Fischbach, Claudia and Schulz, Michaela B. and Goepferich, Achim and Blunk, Torsten (2005) Adipose tissue engineering based on mesenchymal stem cells and basic fibroblast growth factor in vitro. TISSUE ENGINEERING, 11 (11-12). pp. 1840-1851. ISSN 2152-4947,
Full text not available from this repository. (Request a copy)Abstract
Despite the clinical need for reconstructive and plastic surgery, the supply of engineered adipose tissue equivalents still remains a challenge. As yet, only preadipocytes have been applied as a cell material for the in vitro tissue engineering of fat. Herein, we report the establishment of a three-dimensional (3-D) long-term cell culture, using bone marrow-derived mesenchymal stem cells (MSCs) as an alternative cell source and custom-made poly(lactic-co-glycolic acid) ( PLGA) scaffolds as a cell carrier. Cell-polymer constructs were cultivated for 4 weeks in both the absence and presence of basic fibroblast growth factor ( bFGF), which was previously shown to strongly enhance the adipogenesis of MSCs in conventional 2-D short-term culture. A striking enhancement of the adipogenic differentiation of MSCs and tissue development caused by bFGF in the 3-D culture was observed by osmium tetroxide histology and scanning electron microscopy. At the molecular level, reflecting the increased accumulation of lipids, bFGF increased the enzymatic activity of glycerol-3-phosphate dehydrogenase, a late marker of adipogenesis, and the expression of adipocyte-specific genes peroxisome proliferator activated receptor-gamma 2 (PPAR gamma 2) and glucose transporter-4 (GLUT4), as assessed by reverse transcription-polymerase chain reaction. This study demonstrates that the use of bone marrow-derived MSCs, especially in combination with bFGF, may represent a promising approach to adipose tissue engineering.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MARROW STROMAL CELLS; ADIPOCYTE PRECURSOR CELLS; POLYMER SCAFFOLD; LONG-TERM; DIFFERENTIATION; ADIPOGENESIS; CULTURE; VIVO; FAT; REPAIR; |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Apr 2021 04:55 |
| Last Modified: | 26 Apr 2021 04:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35502 |
Actions (login required)
 |
View Item |
