Ferreira-Cerca, Sébastien and Poell, Gisela and Gleizes, P. E. and Tschochner, Herbert and Milkereit, Philipp (2005) Roles of eukaryotic ribosomal proteins in maturation and transport of pre-18S rRNA and ribosome function. MOLECULAR CELL, 20 (2). pp. 263-275. ISSN 1097-2765, 1097-4164
Full text not available from this repository. (Request a copy)Abstract
Despite the rising knowledge about ribosome function and structure and how ribosomal subunits assemble in vitro in bacteria, the in vivo role of many ribosomal proteins remains obscure both in pro- and eukaryotes. Our systematic analysis of yeast ribosomal proteins (r-proteins) of the small subunit revealed that most eukaryotic r-proteins fulfill different roles in ribosome biogenesis, making them indispensable for growth. Different r-proteins control distinct steps of nuclear and cytoplasmic pre-18S rRNA processing and, thus, ensure that only properly assembled ribosomes become engaged in translation. Comparative analysis of dynamic and steady-state maturation assays revealed that several r-proteins are required for efficient nuclear export of pre-18S rRNA, suggesting that they form an interaction platform with the export machinery. In contrast, the presence of other r-proteins is mainly required before nuclear export is initiated. Our studies draw a correlation between the in vitro assembly, structural localization, and in vivo function of r-proteins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE GENOME; ANGSTROM RESOLUTION; NUCLEAR EXPORT; PRE-RIBOSOMES; PEPTIDYLTRANSFERASE ACTIVITY; CRYSTAL-STRUCTURE; YEAST; SUBUNITS; MUTANTS; TRANSLATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Philipp Milkereit |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Apr 2021 05:15 |
| Last Modified: | 26 Apr 2021 05:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35511 |
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