Rausch, Philipp and Basic, Marijana and Batra, Arvind and Bischoff, Stephan C. and Blaut, Michael and Clavel, Thomas and Glaesner, Joachim and Gopalakrishnan, Shreya and Grass, Guntram A. and Guenther, Claudia and Haller, Dirk and Hirose, Misa and Ibrahim, Saleh and Loh, Gunnar and Mattner, Jochen and Nagel, Stefan and Pabst, Oliver and Schmidt, Franziska and Siegmund, Britta and Strowig, Till and Volynets, Valentina and Wirtz, Stefan and Zeissig, Sebastian and Zeissig, Yvonne and Bleich, Andre and Baines, John F. (2016) Analysis of factors contributing to variation in the C57BL/6J fecal microbiota across German animal facilities. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 306 (5). pp. 343-355. ISSN 1438-4221, 1618-0607
Full text not available from this repository. (Request a copy)Abstract
The intestinal microbiota is involved in many physiological processes and it is increasingly recognized that differences in community composition can influence the outcome of a variety of murine models used in biomedical research. In an effort to describe and account for the variation in intestinal microbiota composition across the animal facilities of participating members of the DFG Priority Program 1656 "Intestinal Microbiota", we performed a survey of C57BL/6J mice from 21 different mouse rooms/facilities located at 13 different institutions across Germany. Fresh feces was sampled from five mice per room facility using standardized procedures, followed by extraction and 16S rRNA gene profiling (V1-V2 region, Illumina MiSeq) at both the DNA and RNA (reverse transcribed to cDNA) level. In order to determine the variables contributing to bacterial community differences, we collected detailed questionnaires of animal husbandry practices and incorporated this information into our analyses. We identified considerable variation in a number of descriptive aspects including the proportions of major phyla, alpha- and beta diversity, all of which displayed significant associations to specific aspects of husbandry. Salient findings include a reduction in alpha diversity with the use of irradiated chow, an increase in inter-individual variability (beta diversity) with respect to barrier access and open cages and an increase in bacterial community divergence with time since importing from a vendor. We further observe a high degree of facility-level individuality, which is likely due to each facility harboring its own unique combination of multiple varying attributes of animal husbandry. While it is important to account and control for such differences between facilities, the documentation of such diversity may also serve as a valuable future resource for investigating the origins of microbial-driven host phenotypes. (C) 2016 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY license
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALTERED SCHAEDLER FLORA; REGULATORY T-CELLS; GUT MICROBIOME; ULCERATIVE-COLITIS; RHEUMATOID-ARTHRITIS; MURINE MICROBIOTA; HYDROGEN-SULFIDE; CROHNS-DISEASE; MOUSE MODEL; FREE MICE; Gut microbiota; Mouse husbandry; C57BL/6J; 16S rRNA gene |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2019 11:44 |
| Last Modified: | 05 Apr 2019 11:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3552 |
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