Song, S.-H. and Dick, Bernhard and Zirak, P. and Penzkofer, Alfons and Schiereis, T. and Hegemann, P. (2005) Absorption and emission spectroscopic characterisation of combined wildtype LOV1-LOV2 domain of phot from Chlamydomonas reinhardtii. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 81 (1). pp. 55-65. ISSN 1011-1344,
Full text not available from this repository. (Request a copy)Abstract
An absorption and emission spectroscopic characterisation of the combined wild-type LOV1-LOV2 domain string (abbreviated LOV1/2) of phot from the green alga Chlamydomonas reinhardtii is carried out at pH 8. A LOV1/2-MBP fusion protein (MBP = maltose binding protein) and LOV1/2 with a His-tag at the C-terminus (LOV1/2-His) expressed in an Escherichia coli strain are investigated. Blue-light photo-excitation generates a non-fluorescent intermediate photoproduct (flavin-C(4a)-cysteinyl adduct with absorption peak at 390 nm). The photo-cycle dynamics is studied by dark-state absorption and fluorescence measurement, by following the temporal absorption and emission changes under blue and violet light exposure, and by measuring the temporal absorption and fluorescence recovery after light exposure. The fluorescence quantum yield, OF, of the dark adapted samples is phi(F)(LOV1/2-His) approximate to 0.15 and phi(F)(LOVI/2-MBP) approximate to 0.17. A bi-exponential absorption recovery after light exposure with a fast (in the several 10-s range) and a slow component (in the near 10-min range) are resolved. The quantum yield of photo-adduct formation, phi(Ad), is extracted from excitation intensity dependent absorption measurements. It decreases somewhat with rising excitation intensity. The behaviour of the combined wildtype LOV1-LOV2 double domains is compared with the behaviour of the separate LOV1 and LOV2 domains. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLUE-LIGHT RECEPTOR; MUTATED PHOT-LOV1 DOMAIN; AQUEOUS-SOLUTION; LOV1 DOMAIN; PHOTOCHEMICAL PROPERTIES; FLUORESCENCE BEHAVIOR; BINDING DOMAIN; HIGHER-PLANTS; FLAVIN; PHOTOTROPIN; LOV domain; phototropin; blue-light photoreceptor; Chlamydomonas reinhardtii; absorption spectroscopy; fluorescence spectroscopy; photo-cycle; flavoprotein; FMN; flavin-C(4a)-cysteinyl adduct |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Molecular Spectroscopy and Photochemistry) > Prof. Dr. Bernhard Dick |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Apr 2021 06:59 |
| Last Modified: | 26 Apr 2021 06:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35537 |
Actions (login required)
 |
View Item |
