Cano-Raya, C. and Ramos, M. D. F. and Vallvey, L. F. C. and Wolfbeis, Otto S. and Schaeferling, Michael (2005) Fluorescence quenching of the europium tetracycline hydrogen peroxide complex by copper(II) and other metal ions. APPLIED SPECTROSCOPY, 59 (10). pp. 1209-1216. ISSN 0003-7028, 1943-3530
Full text not available from this repository. (Request a copy)Abstract
The europium-tetracycline complex [Eu(Tc)] is known to show only weak fluorescence with an emission maximum at 615 nm. On addition of hydrogen peroxide (HP), the strongly fluorescent [Eu(Tc)(HP)] complex is formed, which displays a 15-fold stronger luminescence intensity. This study describes the decrease in luminescence intensity of the [Eu(Tc)(HP)] complex in aqueous solution in the presence of Cu2+, Fe3+, Ag+, Al3+, Zn2+, Co2+, Ni2+, Mn2+, Ca2+, and Mg2+. Static and dynamic quenching can be induced by Cu2+, and these processes were quantified by means of their quenching constants. Stern-Volmer plots were also derived from lifetime imaging measurements accomplished by the rapid lifetime determination (RLD) technique based on microwell plate assays, and also by the time-correlated single photon counting (TCSPC) technique. According to those data, a time-resolved fluorescent method for copper determination can be proposed that is based on dynamic quenching of the [Eu(Tc)(HP)l complex by Cu2+ ions. The response to copper concentrations is linear up to 1.6 mu mol L-1, providing a detection limit of 0.2 mu mol L-1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENERGY-TRANSFER; LUMINESCENCE; GLUCOSE; PROBE; TERBIUM(III); LIFETIME; heavy metal quenching; time-resolved fluorescence imaging; single photon counting technique; europium tetracycline hydrogen peroxide ternary complex; copper determination |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Apr 2021 07:35 |
| Last Modified: | 26 Apr 2021 07:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35545 |
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