Hoefelschweiger, Bianca K. and Pfeifer, Lutz and Wolfbeis, Otto S. (2005) Screening scheme based on measurement of fluorescence lifetime in the nanosecond domain. JOURNAL OF BIOMOLECULAR SCREENING, 10 (7). pp. 687-694. ISSN 1087-0571, 1552-454X
Full text not available from this repository. (Request a copy)Abstract
The authors demonstrate that the fluorescence lifetime of certain fluorescent labels is a useful parameter to detect affinity binding between biotin and streptavidin, as well as between biotinylated bovine serum albumin and streptavidin. The assay is performed in a microplate format, and lifetimes are determined using dye laser-induced fluorescence. Four fluorescent labels are presented that undergo a significant change in their lifetime upon affinity binding. The scheme. referred to as the fluorescence lifetime affinity assay, has several attractive features in that it requires single labeling only, represents a homogeneous assay, allows each of the 2 binding partners to be labeled, and is compatible with the standard microwell formats used in hight-throughput screening.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ASSAY; TECHNOLOGIES; SENSORS; BINDING; LABELS; HTS; laser-induced fluorescence; lifetime; fluorescent label; affinity binding; affinity assay; decay time |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Apr 2021 10:04 |
| Last Modified: | 26 Apr 2021 10:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35589 |
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