Nisius, Lydia and Stadler, Max and Kalbitzer, Hans Robert and Brunner, Eike (2005) NMR spectroscopic study of noble gas binding into the engineered cavity of HPr(I14A) from Staphylococcus carnosus. JOURNAL OF PHYSICAL CHEMISTRY B, 109 (38). pp. 17795-17798. ISSN 1520-6106
Full text not available from this repository. (Request a copy)Abstract
Xenon binding into preexisting cavities in proteins is a well-known phenomenon. Here we investigate the interaction of helium, neon, and argon with hydrophobic cavities in proteins by NMR spectroscopy. H-1 and N-15 chemical shifts of the I14A mutant of the histidine-containing phosphocarrier protein (HPr(I14A)) from Staphylococcus carnosus are analyzed by chemical shift mapping. Total noble gas induced chemical shifts, A, are calculated and compared with the corresponding values obtained using xenon as a probe atom. This comparison reveals that the same cavity is detected with both argon and xenon. Measurements using the smaller noble gases helium and neon as probe atoms do not result in comparable effects. The dependence of amide proton and nitrogen chemical shifts on the argon concentration is investigated in the range from 10 mM up to 158 mM. The average dissociation constant for argon binding into the engineered cavity is determined to be about 90 mM.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONTAINING PHOSPHOCARRIER PROTEIN; LIPID TRANSFER PROTEIN; LASER-POLARIZED XENON; XE-129 NMR; HYDROPHOBIC CAVITY; CHEMICAL-SHIFT; SYSTEM; MUTANT; WATER; I14A |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Alumni or Retired > Prof. Dr. Eike Brunner Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Aug 2023 08:03 |
| Last Modified: | 16 Aug 2023 08:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35620 |
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