Nardocci, Gino and Simonet, Nicolas G. and Navarro, Cristina and Laengst, Gernot and Alvarez, Marco (2016) Differential enrichment of TTF-I and Tip5 in the T-like promoter structures of the rDNA contribute to the epigenetic response of Cyprinus carpio during environmental adaptation. BIOCHEMISTRY AND CELL BIOLOGY, 94 (4). pp. 315-321. ISSN 0829-8211, 1208-6002
Full text not available from this repository. (Request a copy)Abstract
To ensure homeostasis, ectothermic organisms adapt to environmental variations through molecular mechanisms. We previously reported that during the seasonal acclimatization of the common carp Cyprinus carpio, molecular and cellular functions are reprogrammed, resulting in distinctive traits. Importantly, the carp undergoes a drastic rearrangement of nucleolar components during adaptation. This ultrastructural feature reflects a fine modulation of rRNA gene transcription. Specifically, we identified the involvement of the transcription termination factor I (TTF-I) and Tip-5 (member of nucleolar remodeling complex, NoRC) in the control of rRNA transcription. Our results suggest that differential Tip5 enrichment is essential for silencing carp ribosomal genes and that the T-0 element is key for regulating the ribosomal gene during the acclimatization process. Interestingly, the expression and content of Tip5 were significantly higher in winter than in summer. Since carp ribosomal gene expression is lower in the winter than in summer, and considering that expression concomitantly occurs with nucleolar ultrastructural changes of the acclimatization process, these results indicate that Tip5 importantly contributes to silencing the ribosomal genes. In conclusion, the current study provides novel evidence on the contributions of TTF-I and NoRC in the environmental reprogramming of ribosomal genes during the seasonal adaptation process in carp.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RIBOSOMAL-RNA GENES; REMODELING COMPLEX NORC; TRANSCRIPTION TERMINATION; SEASONAL ACCLIMATIZATION; POLYMERASE-I; EURYTHERMAL FISH; CHROMATIN; EXPRESSION; PROTEIN; NUCLEOLUS; acclimatization; homeostasis; epigenetics; ribosomal genes; nucleolar remodeling complex |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2019 12:29 |
| Last Modified: | 05 Apr 2019 12:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3567 |
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