Brandl, K. and Glueck, Thomas and Huber, C. and Salzberger, Bernd and Falk, Werner and Hartmann, Pia (2005) TLR-4 surface display on human monocytes is increased in septic patients. EUROPEAN JOURNAL OF MEDICAL RESEARCH, 10 (8). pp. 319-324. ISSN 0949-2321, 2047-783X
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Background: Sepsis is a serious condition, most often occurring as a complication of bacterial infections. The Toll-like receptors (TLR)-2 and TLR-4 have been identified as key molecules in response to Gram-positive and Gram-negative bacteria. This study aimed to assess possible alterations of the surface display of TLR-2 and TLR-4 on monocytes and granulocytes derived from patients with sepsis in comparison with healthy Controls. Methods: We have utilized flow-cytometry to determine the presence of TLR-2 and TLR-4 on the cell surface at baseline and in response to I-PS (40 ng/ml) in vitro. Results: We found no significant differences of TLR-2 display on monocytes and granulocytes from septic patients compared to controls. Surface display of TLR-4 on monocytes from septic patients at baseline was significantly higher than in healthy controls but there was no further response to LPS, whereas controls showed a significant increase of TLR-4 display on the cell surface after LPS stimulation. In contrast, TLR-4 baseline cell surface display on granulocytes was significantly lower in septic patients than in controls and there was no response to LPS in both groups. Conclusion: Our data suggest a complex relationship between TLR-4 display and bacterial challenge in vivo and in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TOLL-LIKE RECEPTORS; DELAYED-HYPERSENSITIVITY; BACTERIAL PEPTIDOGLYCANS; ADJUVANT ACTIVITY; LIPOPOLYSACCHARIDE; SEPSIS; TLR2; TLR4; sepsis; monocytes; granulocytes |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 May 2021 09:02 |
| Last Modified: | 03 May 2021 09:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35769 |
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