Zirak, P. and Penzkofer, Alfons and Schiereis, T. and Hegemann, P. and Jungmann, A. and Schlichting, I. (2005) Absorption and fluorescence spectroscopic characterization of BLUF domain of AppA from Rhodobacter sphaeroides. CHEMICAL PHYSICS, 315 (1-2). pp. 142-154. ISSN 0301-0104, 1873-4421
Full text not available from this repository. (Request a copy)Abstract
The BLUF domain of the transcriptional anti-repressor protein AppA from the non-sulfur anoxyphototrophic purple bacterium Rhodobacter sphaeroides was characterized by absorption and emission spectroscopy. The BLUF domain constructs AppA] 48 (consisting of amino-acid residues 1-148) and AppA(126) (amino-acid residues 1-126) are investigated. The cofactor of the investigated domains is found to consist of a mixture of the flavins riboflavin, FMN, and FAD. The dark-adapted domains exist in two different active receptor conformations (receptor states) with different sub-nanosecond fluorescence lifetimes (BLUFr,f and BLUFr,sl) and a small non-interacting conformation (BLUF,,,). The active receptor conformations are transformed to putative signalling states (BLUFs,f and BLUFs,sl) of low fluorescence efficiency and picosecond fluorescence lifetime by blue-light excitation (light-adapted domains). In the dark at room temperature both signalling states recover back to the initial receptor states with a time constant of about 17 min. A quantum yield of signalling state formation of about 25% was determined by intensity dependent transmission measurements. A photo-cycle scheme is presented including photo-induced charge transfer complex formation, charge recombination, and protein binding pocket reorganisation. (C) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLAVIN ADENINE-DINUCLEOTIDE; PHOTOSYNTHESIS GENE-EXPRESSION; AQUEOUS-SOLUTION; CHLAMYDOMONAS-REINHARDTII; TRANSCRIPTIONAL ANTIREPRESSOR; FEMTOSECOND DYNAMICS; LIGHT PHOTORECEPTOR; QUANTUM YIELD; HIGHER-PLANTS; LOV1 DOMAIN; BLUF domain; AppA; blue-light photoreceptor; Rhodobacter sphaeroides; absorption spectroscopy; fluorescence spectroscopy; fluorescence up-conversion; photo-cycle; flavoprotein; FAD; FMN; riboflavin |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 May 2021 09:37 |
| Last Modified: | 03 May 2021 09:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35776 |
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