Horn, Christian and Steinem, Claudia (2005) Photocurrents generated by bacteriorhodopsin adsorbed on nano-black lipid membranes. BIOPHYSICAL JOURNAL, 89 (2). pp. 1046-1054. ISSN 0006-3495, 1542-0086
Full text not available from this repository. (Request a copy)Abstract
Purple membranes were adsorbed on freestanding lipid bilayers, termed nano- black lipid membranes ( nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano- BLMs were obtained by. first coating the upper surface of the highly ordered porous alumina substrates with a thin gold layer followed by chemisorption of 1,2- dipalmitoyl- sn- glycero- 3- phosphothioethanol and subsequent addition of a droplet of 1,2- diphytanoyl- sn- glycero- 3-phosphocholine and octadecylamine dissolved in n- decane onto the hydrophobic submonolayer. By means of impedance spectroscopy, the quality of the nano- BLMs was verified. The electrical parameters confirm the formation of single lipid bilayers with high membrane resistances covering the porous matrix. Adsorption of purple membranes on the nano- BLMs was followed by recording the photocurrents generated by bacteriorhodopsin upon continuous light illumination. The membrane system exhibits a very high long- term stability with the advantage that not only transient but also stationary currents are recordable. By adding the proton ionophore carbonyl cyanide- m- chlorophenylhydrazone the conductivity of the nano- BLMs increases, resulting in a higher stationary current, which proves that proton conductance occurs across the nano- BLMs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PLANAR BILAYER-MEMBRANES; HEXAGONAL PORE ARRAYS; PHOTOELECTRIC RESPONSE; PURPLE-MEMBRANE; ANODIC ALUMINA; PROTON TRANSLOCATION; ION CHANNELS; THIN-FILMS; PHOTORECEPTOR; FABRICATION; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 May 2021 04:41 |
| Last Modified: | 03 May 2021 04:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35785 |
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