Uhl, J. R. and Vetter, E. A. and Boldt, K. L. and Johnston, B. W. and Ramin, K. D. and Adams, M. J. and Ferrieri, P. and Reischl, Udo and Cockerill, F. R. (2005) Use of the Roche LightCycler Strep B assay for detection of group B streptococcus from vaginal and rectal swabs. JOURNAL OF CLINICAL MICROBIOLOGY, 43 (8). pp. 4046-4051. ISSN 0095-1137, 1098-660X
Full text not available from this repository. (Request a copy)Abstract
The results for a real-time PCR assay, using the LightCycler Strep B analyte-specific reagents (Roche Diagnostics Corporation, Indianapolis, Ind.), were compared to a direct plate method combined with a broth enrichment culture method for detection of group B streptococcus colonization in pregnant women. Two separate evaluations were conducted using two different automated nucleic extraction instruments, the MagNA Pure LC instrument (Roche Diagnostics Corporation) and the lower-capacity MagNA Pure Compact instrument (Roche Diagnostics Corporation). The sensitivities, specificities, and positive and negative predictive values for the different evaluation methods were as follow: for the LightCycler Strep B assay with MagNA Pure LC, 100, 97, 90, and 100%, respectively; for the LightCycler Strep B assay with MagNA Pure Compact, 92.5, 99, 97, and 97.5%, respectively. The LightCycler Strep B assay combined with either MagNA Pure LC or MagNA Pure Compact extraction is a suitable method for detecting group B streptococcus colonization in pregnant women. An advantage of the LightCycler assay over culture is the considerably reduced turnaround time for results.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RAPID DETECTION; PREGNANT-WOMEN; PREVENTION; STRATEGIES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 May 2021 08:17 |
| Last Modified: | 03 May 2021 08:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35818 |
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