Haindl, Markus and Holler, Eggehard (2005) Use of the giant multinucleate plasmodium of Physarum polycephalum to study RNA interference in the myxomycete. ANALYTICAL BIOCHEMISTRY, 342 (2). pp. 194-199. ISSN 0003-2697, 1096-0309
Full text not available from this repository. (Request a copy)Abstract
The plasmodium of Physarum polycephalum harbors billions of synchronized nuclei in a single cell of complex structure. Due to its synchrony and extreme size, it is used as a model to study events oil a single cell level, such as cell cycle and differentiation. We show here for the first time that this model, despite its enormous size and structural complexity, is accessible to RNA interference by simple injection of dsRNA or siRNA. The targeted gene is that of polymalatase, an intracellular adapter of poly(beta-L-malate) involved in the maintenance of the synchrony and functioning as an extracellular hydrolase of this polymer. Real-time reverse transcriptase polymerase chain reaction analysis revealed that the specific mRNA was knocked down to about 10% of the original level. The Suppression of a single injection lasted for approximately 14 cell cycles (144 h) and could be prolonged for ally time by repeated dsRNA injections. Western blots indicated that the knockdown of RNA was paralleled by a strong reduction in polymalatase synthesis. However, a change in the phenotype of the plasmodium Could not be clearly observed. In principle, the plasmodium offers an easy system for studying gene knockdown by RNA interference. (c) 2005 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-CYCLE; GENE; RNA interference; polymalatase; polynucleate cells; plasmodium; microinjection |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Alumni or Retired > Prof. Dr. Eggehard Holler |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 May 2021 07:33 |
| Last Modified: | 04 May 2021 07:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35876 |
Actions (login required)
 |
View Item |
