Kranzer, Katharina and Soellner, Liane and Aigner, Michael and Lehn, Norbert and Deml, Ludwig and Rehli, Michael and Schneider-Brachert, Wulf (2005) Impact of Helicobacter pylori virulence factors and compounds on activation and maturation of human dendritic cells. INFECTION AND IMMUNITY, 73 (7). pp. 4180-4189. ISSN 0019-9567, 1098-5522
Full text not available from this repository. (Request a copy)Abstract
Recently, we and others have shown that Helicobacter pylori induces dendritic cell (DC) activation and maturation. However, the impact of virulence factors on the interplay between DCs and H. pylori remains elusive. Therefore, we investigated the contribution of cag pathogenicity island (PAI) and VacA status on cytokine release and up-regulation of costimulatory molecules in H. pylori-treated DCs. In addition, to characterize the stimulatory capacity of H. pylori compounds in more detail, we studied the effect of formalin-inactivated and sonicated H. pylori, as well as secreted H. pylori molecules, on DCs. Incubation of DCs with viable or formalin-inactivated H. pylori induced comparable secretion of interleukin-6 (IL-6), IL-8, IL-10, IL-12, IL-10, and tumor necrosis factor (TNF). In contrast, IL-12 and IL-1 beta release was significantly reduced in DCs treated with sonicated bacteria and secreted bacterial molecules. Treatment of sonicated H. pylori preparations with polymyxin B resulted in a significant reduction of IL-8 and IL-6 secretion, suggesting that H. pylori-derived lipopolysaccharide at least partially contributes to activation of immature DCs. In addition, the capacity of H. pylori-pulsed DCs to activate allogeneic T cells was not affected by cag PAI and VacA. Pretreatment of DC with cytochalasin D significantly inhibited secretion of IL-12, IL-10, and TNF, indicating that phagocytosis of H. pylori contributes to maximal activation of DCs. Taken together, our results suggest that DC activation and maturation, as well as DC-mediated T-cell activation, are independent of the cag PAI and VacA status of H. pylori.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CAG PATHOGENICITY ISLAND; GASTRIC EPITHELIAL-CELLS; NF-KAPPA-B; VACUOLATING CYTOTOXIN; GASTRODUODENAL DISEASE; INTERLEUKIN-12 PRODUCTION; NEISSERIA-MENINGITIDIS; CYTOKINE PRODUCTION; PEPTIC-ULCERATION; MONGOLIAN GERBIL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2021 08:23 |
| Last Modified: | 07 May 2021 08:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35937 |
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