Todorov, Vladimir T. and Voelkl, Simon and Friedrich, Juergen and Kunz-Schughart, Leoni A. and Hehlgans, Thomas and Vermeulen, L. and Haegeman, G. and Schmitz, M. L. and Kurtz, Armin (2005) Role of CREB1 and NF kappa B-p65 in the down-regulation of renin gene expression by tumor necrosis factor alpha. JOURNAL OF BIOLOGICAL CHEMISTRY, 280 (26). pp. 24356-24362. ISSN 0021-9258,
Full text not available from this repository. (Request a copy)Abstract
Tumor necrosis factor-alpha( TNF alpha) is a potent inhibitor of renin gene expression in renal juxtaglomerular cells. We have found that TNF alpha suppresses renin transcription via transcription factor NF kappa B, which targets a cAMP responsive element ( CRE) in the renin promoter. Here we aimed to further clarify the role of NF kappa B and the canonical CRE- binding proteins of the CRE- binding protein/ activating transcription factor ( CREB/ ATF) family in the inhibition of renin gene expression by TNF alpha in the juxtaglomerular cell line As4.1. TNF alpha caused a moderate decrease in the binding of CREB1 to its cognate CRE DNA binding site. On the other hand, NF kappa B- p65 transcriptional activity was substantially reduced by TNF alpha, which targeted a trans- activation domain at the very C terminus of the p65 molecule. Our results suggest that TNF alpha inhibits renin gene expression by decreasing the transactivating capacity of NF kappa B- p65 and partially by attenuating CREB1 binding to CRE.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; CAMP-RESPONSIVE ELEMENT; BINDING PROTEIN CREB; TRANSCRIPTION FACTORS; P65 SUBUNIT; DEPENDENT TRANSCRIPTION; JUXTAGLOMERULAR CELLS; SIGNAL-TRANSDUCTION; ENHANCER ELEMENTS; ZETA-PKC; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie Medicine > Lehrstuhl für Pathologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2021 09:55 |
| Last Modified: | 07 May 2021 09:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35942 |
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