Franzen, Caspar and Fischer, Susanne and Schroeder, Josef and Bleiss, Wilrid and Schneuwly, Stephan and Schoelmerich, Juergen and Salzberger, Bernd (2005) In vitro cultivation of an insect microsporidian Tubulinosema ratisbonensis in mammalian cells. JOURNAL OF EUKARYOTIC MICROBIOLOGY, 52 (4). pp. 349-355. ISSN 1066-5234,
Full text not available from this repository. (Request a copy)Abstract
Tubulinosenza ratisbonensis is a microsporidian pathogen of Drosophila melanogaster belonging to the family Tubulinosematidae. The microsporidia in this family mainly cause infections in invertebrate hosts, but two members of this family, Brachiolo vesicidarum and Brachiola algerae, have been found to cause infections in humans as well. Moreover, B. algerae call be transmitted to immunodeficient mice and grows in mammalian cell cultures. Thus, the examination of the opportunistic properties of other members of the family Tubulinosematidae is important. Spores of T. ratisbonensis, isolated from infected fruit flies, were used to inoculate mammalian and insect cell cultures. Parasite growth was only seen in human lung fibroblasts. No growth was seen in Vero cells or insect cell cultures. Comparison of growth kinetics at 31 degrees C and 37 degrees C showed that there were fewer and smaller parasitic foci in Cultures incubated at 37 degrees C. Transmission electron microscopy revealed the typical ultrastructure of T ratisbonensis, and scanning electron microscopy showed oval or slightly pyriform spores, with some spores having extruded their polar tubes. The PCR-amplified sequences of rDNA fragments from infected cell cultures were 100% identical to the original T. ratisbonensis rRNA sequence. As T. ratisbonensis is able to proliferate in mammalian cell Cultures, it may have the opportunistic properties of other members of the family Tubulinosematidae.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NOSEMA-ALGERAE; DROSOPHILA-MELANOGASTER; BRACHIOLA-ALGERAE; PARASITE; MYOSITIS; CULTURE; MICE; in vitro culture; microsporidia; Tubulinosematidae |
| Subjects: | 500 Science > 590 Zoological sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie Biology, Preclinical Medicine > Institut für Zoologie > Entwicklungsbiologie (Prof. Dr. Stephan Schneuwly) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2021 10:47 |
| Last Modified: | 07 May 2021 10:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35947 |
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