Stadtherr, Karin and Wolf, Hans and Lindner, Petra (2005) An aptamer-based protein biochip. ANALYTICAL CHEMISTRY, 77 (11). pp. 3437-3443. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
The establishment of an aptamer-based biochip for protein detection is described. Using a model system comprising human IgE as the analyte and single-stranded DNA aptamers specific for IgE or anti-IgE antibodies as immobilized ligands on chips, we could demonstrate that aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. Aptamer-based analyte detection on glass slides could clearly be demonstrated at minimum concentrations of 10 ng/mL IgE. In addition, we successfully showed specific analyte recognition in complex protein samples by the aptamerbased biochip system. Using DNA aptamers specific for human thrombin as an additional model receptor/ligand system, dual protein detection on a single slide could be proven. In conclusion, we could show the suitability of nucleic acid aptamers as low molecular weight receptors on biochips for sensitive and specific protein detection, representing an innovative tool for future proteomics.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGH-AFFINITY; ARRAYS; LIGANDS; BIOSENSOR; THROMBIN; RNA; ELECTROPHORESIS; MOLECULES; SELECTION; BINDING; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 May 2021 10:11 |
| Last Modified: | 10 May 2021 10:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36016 |
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