von Walter, M. and Rueger, M. and Ragoss, C. and Steffens, G. C. M. and Hollander, D. A. and Paar, O. and Maier, H. R. and Jahnen-Dechent, W. and Bosserhoff, Anja Kathrin and Erli, H. J. (2005) In vitro behavior of a porous TiO2/perlite composite and its surface modification with fibronectin. BIOMATERIALS, 26 (16). pp. 2813-2826. ISSN 0142-9612, 1878-5905
Full text not available from this repository. (Request a copy)Abstract
In this study, we introduce a porous composite material, termed "Ecopore", and describe in vitro investigation of the material and its modification with fibronectin. The material is a sintered compound of rutile TiO2 and the volcanic silicate perlite with a macrostructure of interconnecting pores. It is both inexpensive and easy to manufacture. We first investigated Ecopore for corrosion and leaching of elements in physiological saline. The corrosion supernatants did not contain critical concentrations of toxic trace elements. In an in vitro model, human primary osteoblasts (HOB) were cultured directly on Ecopore. HOB grew on the composite as well as on samples of its single constituents, TiO2 and perlite glass, and remained vital, but cellular spreading was less than on tissue culture plastic. The pro-inflammatory cytokines IL-1 and TNF-alpha were below detection limits in HOB culture supernatants, whereas IL-6 was detectable on a low level. To enhance cellular attachment and growth, the surface of the composite was modified by etching, functionalization with aminosilane and coupling of fibronectin. This modification greatly enhanced the spreading of HOB, indicated by vital staining and Sodium 3'-[1-(phenylaminocarbonyl)-3,4-tetrazoliuml-bis (4-methoxy-6-nitro) benzene sulfortic acid hydrate (XTT) metabolism assays. HOB grew on the entire visible surface of porous fibronectin-modified composite, expressing alkaline phosphatase. a mature osteoblast marker. We conclude that Ecopore is non-toxic and sustains HOB growth, cellular spreading being improvable by coating with fibronectin. The composite may be usable in the field of bone substitution. (C) 2004 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TITANIUM; CELLS; MOLECULES; IMPLANTS; CULTURES; ACID; ceramic; cytotoxicity; surface modification; fibronectin; osteoblast |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 May 2021 10:23 |
| Last Modified: | 10 May 2021 10:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36026 |
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