Craig, Dogulas B. and Wetzl, Bianca K. and Duerkop, Axel and Wolfbeis, Otto S. (2005) Determination of picomolar concentrations of proteins using novel amino reactive chameleon labels and capillary electrophoresis laser-induced fluorescence detection. ELECTROPHORESIS, 26 (11). pp. 2208-2213. ISSN 0173-0835,
Full text not available from this repository. (Request a copy)Abstract
Py-1 and Py-6 are novel amino-reactive fluorescent reagents. The names given to them reflect that they consist of a pyrylium group attached to small aromatic moieties. Upon reaction with a primary amine there is a large spectral shift in the reagent, rendering them effectively fluorogenic. In this study, these reagents were used to label a test protein, (human serum albumin), and the sample was analyzed by capillary electrophoresis and laser-induced fluorescence detection. Detection limits after a 60 min labeling reaction at 22 degrees C (Py-1) and 50 degrees C (Py-6) were 6.5 ng/mL (98 pm) for Py-1 and 1.2 ng/mL (18 pm) for Py-6. Separation of immunoglobulin G (IgG), human serum albumin, lipase, and myoglobin after labeling with Py-6 were performed. The method was further modified to make it amenable to automation. Unlike many other amino reactive reagents used to label protein amino groups, reaction with Py-1 and Py-6 do not alter the charge of the protein and the advantage of this with respect to electrophoretic separations is discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NONCOVALENT; PRECOLUMN; ASSAY; NM; capillary electrophoresis; chameleon dyes; laser-induced fluorescence |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 May 2021 12:10 |
| Last Modified: | 10 May 2021 12:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36037 |
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