Jeschke, M. G. and Rensing, H. and Klein, Dagmar and Schubert, Thomas and Mautes, AEM and Bolder, Ulrich and Croner, R. S. (2005) Insulin prevents liver damage and preserves liver function in lipopolysaccharide-induced endotoxemic rats. JOURNAL OF HEPATOLOGY, 42 (6). pp. 870-879. ISSN 0168-8278,
Full text not available from this repository. (Request a copy)Abstract
Background/Aims: Liver integrity and function are crucial for survival of patients suffering from trauma, operations or infections. Insulin decreased mortality and prevented the incidence of multi organ failure and infection in critically ill patients. The aim of the present study was to determine whether insulin exerts positive effects on hepatic homeostasis and function during endotoxemia. Methods: Endotoxemic rats received either saline or insulin. Hepatic morphology and function was determined by measuring the effect of insulin on liver proteins, enzymes, hepatocyte apoptosis and proliferation including caspases-3 and -9 and Bcl-2. Intrahepatic ATP, glucose and lactate concentration were determined by bioluminescence. To determine possible molecular changes the effect of insulin on hepatic cytokine mRNA and gene profile analysis were assessed. Results: Insulin significantly improved hepatic protein synthesis by increasing albumin and decreasing c-reactive protein, P < 0.05. Insulin attenuated hepatic damage by decreasing AST and ALT, P < 0.05. Improved liver morphology was due to decreased hepatocyte apoptosis along with decreased caspase-3 concentration and increased hepatocyte proliferation along with Bcl-2 concentration, P<0.05. Insulin decreased hepatic IL-1 beta, IL-6 and MIF mRNA and improved hepatic glucose metabolism and glycolysis, P < 0.05. GeneChip analysis revealed an anti-inflammatory effect of insulin. Conclusions: Insulin improves hepatic integrity, hepatic glucose metabolism and hepatic function by increasing cell survival and attenuating the hepatic inflammatory response in endotoxemic rats. (C) 2005 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYSTEMIC INFLAMMATORY RESPONSE; NF-KAPPA-B; TREATMENT IMPROVES; MONONUCLEAR-CELLS; OXIDATIVE STRESS; ALBUMIN LEVEL; SEVERE TRAUMA; SEVERE SEPSIS; SEPTIC SHOCK; EXPRESSION; sepsis; inflammation; acute phase response; cytokines |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2021 08:44 |
| Last Modified: | 11 May 2021 08:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36069 |
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