Wallner, Stefan and Schroeder, Christopher and Leitao, Elsa and Berulava, Tea and Haak, Claudia and Beisser, Daniela and Rahmann, Sven and Richter, Andreas S. and Manke, Thomas and Boenisch, Ulrike and Arrigoni, Laura and Froehler, Sebastian and Klironomos, Filippos and Chen, Wei and Rajewsky, Nikolaus and Mueller, Fabian and Ebert, Peter and Lengauer, Thomas and Barann, Matthias and Rosenstiel, Philip and Gasparoni, Gilles and Nordstroem, Karl and Walter, Joern and Brors, Benedikt and Zipprich, Gideon and Felder, Baerbel and Klein-Hitpass, Ludger and Attenberger, Corinna and Schmitz, Gerd and Horsthemke, Bernhard (2016) Epigenetic dynamics of monocyte-to-macrophage differentiation. EPIGENETICS & CHROMATIN, 9: 33. ISSN 1756-8935,
Full text not available from this repository. (Request a copy)Abstract
Background: Monocyte-to-macrophage differentiation involves major biochemical and structural changes. In order to elucidate the role of gene regulatory changes during this process, we used high-throughput sequencing to analyze the complete transcriptome and epigenome of human monocytes that were differentiated in vitro by addition of colony-stimulating factor 1 in serum-free medium. Results: Numerous mRNAs and miRNAs were significantly up-or down-regulated. More than 100 discrete DNA regions, most often far away from transcription start sites, were rapidly demethylated by the ten eleven translocation enzymes, became nucleosome-free and gained histone marks indicative of active enhancers. These regions were unique for macrophages and associated with genes involved in the regulation of the actin cytoskeleton, phagocytosis and innate immune response. Conclusions: In summary, we have discovered a phagocytic gene network that is repressed by DNA methylation in monocytes and rapidly de-repressed after the onset of macrophage differentiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVE DNA DEMETHYLATION; CPG ISLANDS; METHYLATION; TRANSCRIPTION; PHAGOCYTOSIS; EPIGENOMICS; EXPRESSION; SIGNATURES; MICRORNAS; ENHANCERS; Monocyte; Macrophage; Epigenetics; Methylation; Enhancer; Next-generation sequencing; Ten eleven translocation methylcytosine dioxygenase; TET; DEEP; IHEC |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2019 11:40 |
| Last Modified: | 03 Apr 2019 11:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3612 |
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