Wu, Meng and Lin, Zihong and Schaeferling, Michael and Duerkop, Axel and Wolfbeis, Otto S. (2005) Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe. ANALYTICAL BIOCHEMISTRY, 340 (1). pp. 66-73. ISSN 0003-2697, 1096-0309
Full text not available from this repository. (Request a copy)Abstract
A method for optical imaging of the activity of glucose oxidase (GOx) using a fluorescent europium(III) tetracycline probe for hydrogen peroxide is presented. A decay time in the microsecond range and the large Stokes shift of 210 nm of the probe facilitate intensity-based, time-resolved, and decay-time-based imaging of glucose oxidase. Four methods for imaging the activity of GOx were compared, and rapid lifetime determination imaging was found to be the best in giving a linear range from 0.32 to 2.7 mUnit/mL. The detection limit is 0.32 mUnit/mL (1.7 ng mL(-1)) which is similar to that of the time-resolved (gated) imaging using a microtiterplate reader. Fluorescent imaging of the activity of GOx is considered to be a useful toot for GOx-based immunoassays with potential for high-throughput screening, immobilization studies, and biosensor array technologies. (c) 2005 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHEMILUMINESCENCE MICROSCOPY; LUMINESCENCE; BIOSENSOR; ENZYMES; ACID; VISUALIZATION; SPECTROSCOPY; NANOPARTICLE; IMMUNOASSAY; DIAGNOSTICS; glucose oxidase; enzyme imaging; fluorescent probe; time-resolved imaging; enzyme activity assay |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 May 2021 05:58 |
| Last Modified: | 14 May 2021 05:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36148 |
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