Lieb, E and Hacker, M. and Tessmar, Joerg and Kunz-Schughart, Leoni A. and Fiedler, J. and Dahmen, C. and Hersel, U. and Kessler, H. and Schulze, M. B. and Goepferich, Achim (2005) Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides. BIOMATERIALS, 26 (15). pp. 2333-2341. ISSN 0142-9612, 1878-5905
Full text not available from this repository. (Request a copy)Abstract
One promising strategy to control the interactions between biomaterial surfaces and attaching cells involves the covalent grafting of adhesion peptides to polymers on which protein adsorption, which mediates unspecific cell adhesion, is essentially suppressed. This study demonstrates a surface modification concept for the covalent anchoring of RGD peptides to reactive diblock copolymers based on monoamine poly(ethylene glycol)-block-POIY(D,L-lactic acid) (H2N-PEG-PLA). Films of both the amine-reactive (ST-NHPEG(2)PLA(20)) and the thiol-reactive derivative (MP-NH-PEG(2)PLA(40)) were modified with cyclic alphavbeta3/alphavbeta5 integrin subtype specific RGD peptides simply by incubation of the films with buffered solutions of the peptides. Human osteoblasts known to express these integrins were used to determine cell-polymer interactions. The adhesion experiments revealed significantly increased cell numbers and cell spreading on the RGD-modified surfaces mediated by RGD-integrin-interactions. (C) 2004 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OSTEOBLAST-LIKE CELLS; IN-VITRO; POLYMER SCAFFOLDS; BONE-FORMATION; HYDROGELS; DIFFERENTIATION; BIOMATERIALS; SURFACES; PENTAPEPTIDES; MODULATION; cell adhesion; RGD peptide; integrin; osteoblast; surface modification; biomimetic material; poly(lactic acid); poly(ethylene oxide); diblock copolymer |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Pathologie Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 May 2021 06:48 |
| Last Modified: | 14 May 2021 06:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36158 |
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