Schrader, Alexandra and Meyer, Katharina and Walther, Neele and Stolz, Ailine and Feist, Maren and Hand, Elisabeth and von Bonin, Frederike and Evers, Maurits and Kohler, Christian and Shirneshan, Katayoon and Vockerodt, Martina and Klapper, Wolfram and Szczepanowski, Monika and Murray, Paul G. and Bastians, Holger and Truemper, Lorenz and Spang, Rainer and Kube, Dieter (2016) Identification of a new gene regulatory circuit involving B cell receptor activated signaling using a combined analysis of experimental, clinical and global gene expression data. ONCOTARGET, 7 (30). pp. 47061-47081. ISSN 1949-2553,
Full text not available from this repository. (Request a copy)Abstract
To discover new regulatory pathways in B lymphoma cells, we performed a combined analysis of experimental, clinical and global gene expression data. We identified a specific cluster of genes that was coherently expressed in primary lymphoma samples and suppressed by activation of the B cell receptor (BCR) through aIgM treatment of lymphoma cells in vitro. This gene cluster, which we called BCR. 1, includes numerous cell cycle regulators. A reduced expression of BCR. 1 genes after BCR activation was observed in different cell lines and also in CD10(+) germinal center B cells. We found that BCR activation led to a delayed entry to and progression of mitosis and defects in metaphase. Cytogenetic changes were detected upon long-term aIgM treatment. Furthermore, an inverse correlation of BCR. 1 genes with c-Myc co-regulated genes in distinct groups of lymphoma patients was observed. Finally, we showed that the BCR. 1 index discriminates activated B cell-like and germinal centre B cell-like diffuse large B cell lymphoma supporting the functional relevance of this new regulatory circuit and the power of guided clustering for biomarker discovery.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; BURKITTS-LYMPHOMA; INDUCED APOPTOSIS; DIFFERENTIAL EXPRESSION; PATHWAY ACTIVATION; MEDIATED APOPTOSIS; CYCLE REGULATION; IN-VITRO; MITOSIS; TARGET; lymphoma; B cell receptor signaling; guided clustering; cell cycle delay; chromosomal aberrations |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Apr 2019 08:19 |
| Last Modified: | 08 Apr 2019 08:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3617 |
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