Poser, Ina and Rothhammer, Tanja and Dooley, Steven and Weiskirchen, Ralf and Bosserhoff, Anja Katrin (2005) Characterization of Sno expression in malignant melanoma. INTERNATIONAL JOURNAL OF ONCOLOGY, 26 (5). pp. 1411-1417. ISSN 1019-6439, 1791-2423
Full text not available from this repository. (Request a copy)Abstract
Normal cells are controlled by several exocrine factors, whereas tumor cells often lose control by antiproliferative stimuli. It is known that melanoma cells produce transforming growth factor (TGF) beta 1, 2 and 3, but do not respond with growth inhibition. Recently, the Smad inhibitor Ski was found to play a role in this process. We originally analyzed Ski expression in nine melanoma cell lines, however, only one cell line (SK Mel 28) was positive. As all nine cell lines were unresponsive to TGF-beta, we continued to search for the responsible mechanism. Sequencing of the TGF-beta-receptor II, known to be mutated in other kinds of cancer, did not reveal any mutation. A family member of Ski, the proto-oncouene Sno was strongly expressed in all melanoma cell lines on RNA and protein level, but not in melanocytes. To confirm functional relevance of this observation, we used stable antisense Sno transfection for the generation of cell clones with reduced Sno expression. These cell clones displayed reduced cell proliferation, indicating participation of Sno in the escape of melanoma cells from TGF-beta dependent growth control. Searching for TGF-beta target genes that are under control of Sno interference, Id I but not antiproliferative genes p2l, p15, p57 and p27 was identified in the cell clones after antisense Sno expression. In summary, constitutive Sno expression was identified as an important mechanism to shut off antiproliferative TGF-beta signaling in malignant melanoma.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSFORMING-GROWTH-FACTOR; ONCOGENIC PROTEIN SKI; FACTOR-BETA; ONCOPROTEIN SKI; IN-VITRO; RECEPTOR; REPRESSION; SMAD2; CELLS; PROLIFERATION; Sno; melanoma; transforming growth factor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 May 2021 09:33 |
| Last Modified: | 14 May 2021 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36177 |
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