Gruber, Michaela and Wetzl, Bianca and Oswald, Bernhard and Enderlein, Joerg and Wolfbeis, Otto S. (2005) A new fluorescence resonance energy transfer pair and its application to oligonucleotide labeling and fluorescence resonance energy transfer hybridization studies. JOURNAL OF FLUORESCENCE, 15 (3). pp. 207-214. ISSN 1053-0509,
Full text not available from this repository. (Request a copy)Abstract
We describe two new fluorescence resonance energy transfer (FRET) compatible labels, their covalent linkage to oligonucleotides, and their use as donor and acceptor, respectively, in FRET hybridization studies. The dyes belong to the cyanine dyes, and water solubility is imparted by a phosphonate which represents a new solubilizing group in DNA labels. They were linked to amino-modified synthetic oligonucleotides via oxysuccinimide (OSI) esters. The studies performed include binding assays, determinations of molecular distances, homogeneous competitive assays, and limits of detection, which are in the order of 5 pmol/L for a 15-mer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MOLECULAR BEACONS; PROBES; fluorescence hybridization assay; fluorescence resonance energy transfer; long-wavelength fluorescent labels |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 May 2021 09:32 |
| Last Modified: | 14 May 2021 09:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36184 |
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