Pfeifer, Lutz and Stein, K. and Fink, U. and Welker, A. and Wetzl, Bianca and Bastian, P. and Wolfbeis, Otto S. (2005) Improved routine bio-medical and bio-analytical online fluorescence measurements using fluorescence lifetime resolution. JOURNAL OF FLUORESCENCE, 15 (3). pp. 423-432. ISSN 1053-0509, 1573-4994
Full text not available from this repository. (Request a copy)Abstract
Fluorescence techniques are widely used as sensitive detection methods in bio-analytics. The use of the bio-physical parameter fluorescence lifetime additional to the spectral characteristics of fluorescence has the potential to improve fluorescence-related detection methods in terms of selectivity in signal recognition, robustness against disturbing influences, and the accessibility of novel bio-chemical process parameters. This article describes the technical set up of a time-resolving instrument with either a fixed time-gated detection principle for improved evaluation of tissue metabolism by an online monitoring of the tissue autofluorescence or a direct fluorescence lifetime detection principle for lifetime-based fluorescent assays.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LASER-INDUCED FLUORESCENCE; TISSUE; SPECTROSCOPY; ULTRAVIOLET; NAD(P)H; VIVO; NADH; time-resolved fluorescence; fluorescence lifetime; fluorescence assay; autofluorescence; metabolism |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 May 2021 09:33 |
| Last Modified: | 14 May 2021 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36185 |
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