Trapp, S. and von Einem, J. and Hofmann, Helga and Koestler, Josef and Wild, Jens and Wagner, Ralf and Beer, M. and Osterrieder, N. (2005) Potential of equine herpesvirus 1 as a vector for immunization. JOURNAL OF VIROLOGY, 79 (9). pp. 5445-5454. ISSN 0022-538X, 1098-5514
Full text not available from this repository. (Request a copy)Abstract
Key problems using viral vectors for vaccination and gene therapy are antivector immunity, low transduction efficiencies, acute toxicity, and limited capacity to package foreign genetic information. It could be demonstrated that animal and human cells were efficiently transduced with equine herpesvirus 1 (EHV-1) reconstituted from viral DNA maintained and manipulated in Escherichia coli. Between 13 and 23% of primary human CD3(+), CD4(+), CD8(+), CD11b(+), and CD19(+) cells and more than 70% of CD4(+) MT4 cells or various human tumor cell lines (MeWo, Huh7, HeLa, 293T, or H1299) could be transduced with one infectious unit of ERV-1 per cell. After intranasal instillation of EHV-1 into mice, efficient transgene expression in lungs was detectable. Successful immunization using ERV-1 was shown after delivery of the human immunodeficiency virus type I Pr55(gag) precursor by the induction of a Gag-specific CD8+ immune response in mice. Because EHV-1 was not neutralized by human sera containing high titers of antibodies directed against human herpesviruses 1 to 5, it is concluded that this animal herpesvirus has enormous potential as a vaccine vector, because it is able to efficiently transduce a variety of animal and human cells, has high DNA packaging capacity, and can conveniently be maintained and manipulated in prokaryotic cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SIMPLEX-VIRUS TYPE-1; BACTERIAL ARTIFICIAL CHROMOSOME; CYTOTOXIC T-LYMPHOCYTES; IMMEDIATE-EARLY PROTEIN; GENE-THERAPY; NEUTRALIZING ANTIBODIES; RECOMBINANT ADENOVIRUS; VACCINIA VIRUS; GLYCOPROTEIN-C; HIV-1 ISOLATE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 May 2021 10:19 |
| Last Modified: | 14 May 2021 10:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36194 |
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