Bollheimer, L. Cornelius and Wrede, Christian E. and Rockmann, Felix and Ottinger, Iris and Schoelmerich, Juergen and Buettner, Roland (2005) Glucagon production of the rat insulinoma cell line INS-1 - A quantitative comparison with primary rat pancreatic islets. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 330 (1). pp. 327-332. ISSN 0006-291X,
Full text not available from this repository. (Request a copy)Abstract
The rat insulinoma cell line INS-1 is the most commonly used clonal cell model in pancreatic beta-cell research. Considering the multihormonality of many insulinomas we examined as to how INS-1 cells comply with the notion of resembling a pure P-cell line. Glucagon immunoassays revealed that INS-1 cells secrete glucagon in a similar range as islets. By immunohistochemistry we detected a cytoplasmic glucagon signal in INS-1 cells which colocalized with C-peptide. Cellular content of preproglucagon-mRNA and glucagon protein in INS-1 cells was less than two percent of the respective values in islets, which probably reflects differences in the intracellular metabolism and/or secretory pathways. Taken together, it is obvious that INS-1 cells do not represent an exclusively insulin producing beta-cell line. (c) 2005 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BETA-CELL; ALPHA-CELLS; PROINSULIN BIOSYNTHESIS; GENE-EXPRESSION; SECRETION; GLUCOSE; METABOLISM; RELEASE; TUMORS; INS-1; insulinoma; islet; glucagon; preproglucagon-mRNA; free fatty acids |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2021 04:39 |
| Last Modified: | 17 May 2021 04:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36242 |
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