Engelhardt, Maren and Bogdahn, Ulrich and Aigner, Ludwig (2005) Adult retinal pigment epithelium cells express neural progenitor properties and the neuronal precursor protein doublecortin. BRAIN RESEARCH, 1040 (1-2). pp. 98-111. ISSN 0006-8993, 1872-6240
Full text not available from this repository. (Request a copy)Abstract
The adult mammalian retina is devoid of any detectable neurogenesis. However, different cell types have been suggested to potentially act as neural progenitors in the adult mammalian retina in vitro, such as ciliary body (CB), Muller glia, and retinal pigment epithelium (RPE) cells. In rodents and humans, strong evidence for neural stem or progenitor properties exists only for CB-derived cells, but not for other retinal cell types. Here, we provide a comparative analysis of adult rat CB- and RPE-derived cells suggesting that the two cell types share certain neural progenitor properties in vitro. CB and RPE cells expressed neural progenitor markers such as Nestin, Flk-1, Hes1, and Musashi. They proliferated under adherent and neurosphere conditions and showed limited self-renewal. Moreover, they differentiated into neuronal and glial cells based on the expression of differentiation markers such as the young neuronal marker beta-III tubulin and the glial and progenitor markers GFAP and NG2. Expression of beta-III tubulin was found in cells with neuronal and non-neuronal morphology. A subpoptilation of RPE- and C13-derived progenitor cells expressed the neurogenesis-specific protein doublecortin (DCX). Interestingly, DCX expression defined a 411 tubulin-positive CB and P-PE fraction with a distinct neuronal morphology. In summary, the data suggest that RPE cells share with CB cells the potential to de-differentiate into a cell type with neural progenitor-like identity. In addition, DCX expression might define the neuronal-differentiating RPE- and C13-derived progenitor population. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROTUBULE-ASSOCIATED PROTEIN; CENTRAL-NERVOUS-SYSTEM; III BETA-TUBULIN; STEM-CELLS; REGENERATION INVIVO; VERTEBRATE RETINA; EYE DEVELOPMENT; MAMMALIAN EYE; OWL MONKEY; RAT RETINA; RPE; differentiation; neuronal precursors; ciliary body; eye |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2021 06:32 |
| Last Modified: | 17 May 2021 06:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36263 |
Actions (login required)
 |
View Item |
