Karczmarczyk, Aleksandra and Dubiak-Szepietowska, Monika and Vorobii, Mariia and Rodriguez-Emmenegger, Cesar and Dostalek, Jakub and Feller, Karl-Heinz (2016) Sensitive and rapid detection of aflatoxin M1 in milk utilizing enhanced SPR and p(HEMA) brushes. BIOSENSORS & BIOELECTRONICS, 81. pp. 159-165. ISSN 0956-5663, 1873-4235
Full text not available from this repository.Abstract
The rapid and sensitive detection of aflatoxin M-1 (AFM(1)) in milk by using surface plasmon resonance (SPR) biosensor is reported. This low molecular weight mycotoxin is analyzed using an indirect competitive immunoassay that is amplified by secondary antibodies conjugated with Au nanoparticles. In order to prevent fouling on the sensor surface by the constituents present in analyzed milk samples, an interface with poly(2-hydroxyethyl methacrylate) p(HEMA) brush was employed. The study presents a comparison of performance characteristics of p(HEMA)-based sensor with a regularly used polyethylene glycol -based architecture relying on mixed thiol self-assembled monolayer. Both sensors are characterized in terms of surface mass density of immobilized AFM(1) conjugate as well as affinity bound primary and secondary antibodies. The efficiency of the amplification strategy based on Au nanoparticle is discussed. The biosensor allowed for highly sensitive detection of AFM(1) in milk with a limit of detection (LOD) as low as 18 pg mL(-1) with the analysis time of 55 min. (C) 2016 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACE-PLASMON RESONANCE; BLOOD-PLASMA; ENZYME-IMMUNOASSAY; M-1; PRODUCTS; BIOSENSOR; NANOPARTICLES; ELLIPSOMETRY; MYCOTOXINS; ADSORPTION; Surface plasmon resonance; Aflatoxin; Polymer brushes; Gold nanoparticles; Inhibition immunoassay; Signal amplification |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Apr 2019 12:04 |
| Last Modified: | 02 Apr 2019 12:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3627 |
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