Jung, O and Brandes, RP and Kim, IH and Schweda, F and Schmidt, R and Hammock, BD and Busse, R and Fleming, I (2005) Soluble epoxide hydrolase is a main effector of angiotensin II-induced hypertension. HYPERTENSION, 45 (4). pp. 759-765. ISSN 0194-911X, 1524-4563
Full text not available from this repository. (Request a copy)Abstract
The soluble epoxide hydrolase (sEH) metabolizes vasodilatory epoxyeicosatrienoic acids (EETs) to their di-hydroxy derivatives. We hypothesized that the metabolism of EETs by the sEH contributes to angiotensin II-induced hypertension and tested the effects of a water-soluble sEH inhibitor, 12-(3-adamantan-1-yl-ureido) dodecanoic acid (AUDA) on blood pressure. AUDA (130 mu g/mL in drinking water) did not affect blood pressure in normotensive animals but markedly lowered it in mice with angiotensin II-induced hypertension (1 mg/kg per day). The effect of AUDA was accompanied by an increase in urinary salt and water excretion. Intravenous application of AUDA (8 mg/kg) acutely lowered blood pressure and heart rate in animals with angiotensin II-induced hypertension but failed to affect blood pressure in animals with phenylephrine-induced hypertension (29 mg/kg per day). AUDA (0.1 mu mol/L) selectively lowered vascular resistance in an isolated perfused kidney preparation from angiotensin II-pretreated mice but not from control mice. In the perfused hind limb and in isolated carotid arteries from angiotensin II-treated mice, AUDA was without effect. The omega-hydroxylase inhibitor N-methylsulfonyl-12,12-dibromododec-11-enamide, which attenuates formation of the potent vasoconstrictor 20-hydroxyeicosatetraenoic acid, decreased tone in carotid arteries from angiotensin II-treated but not from control mice. These data demonstrate that the decrease in blood pressure observed after sEH inhibition in angiotensin II-induced hypertension can be attributed to an initial reduction in heart rate followed by pressure diuresis resulting from increased perfusion of the kidney. Direct vasodilatation of resistance arteries in skeletal muscles does not appear to contribute to the antihypertensive effects of sEH inhibition in mice.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EPOXYEICOSATRIENOIC ACID METABOLISM; HYPERPOLARIZING FACTOR; ENDOTHELIAL-CELLS; ARACHIDONIC-ACID; NITRIC-OXIDE; CYTOCHROME-P450 METABOLITES; CORONARY-ARTERIES; BLOOD-PRESSURE; KNOCKOUT MICE; INHIBITION; angiotensin; lipids |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Frank Schweda |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2021 13:05 |
| Last Modified: | 17 May 2021 13:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36302 |
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