Chen, Yung-Chia Ariel and Stuwe, Evelyn and Luo, Yicheng and Ninova, Maria and Le Thomas, Adrien and Rozhavskaya, Ekaterina and Li, Sisi and Vempati, Sivani and Laver, John D. and Patel, Dinshaw J. and Smibert, Craig A. and Lipshitz, Howard D. and Toth, Katalin Fejes and Aravin, Alexei A. (2016) Cutoff Suppresses RNA Polymerase II Termination to Ensure Expression of piRNA Precursors. MOLECULAR CELL, 63 (1). pp. 97-109. ISSN 1097-2765, 1097-4164
Full text not available from this repository. (Request a copy)Abstract
Small non-coding RNAs called piRNAs serve as guides for an adaptable immune system that represses transposable elements in germ cells of Metazoa. In Drosophila the RDC complex, composed of Rhino, Deadlock and Cutoff (Cuff) bind chromatin of dual-strand piRNA clusters, special genomic regions, which encode piRNA precursors. The RDC complex is required for transcription of piRNA precursors, though the mechanism by which it licenses transcription remained unknown. Here, we show that Cuff prevents premature termination of RNA polymerase II. Cuff prevents cleavage of nascent RNA at poly(A) sites by interfering with recruitment of the cleavage and polyadenylation specificity factor (CPSF) complex. Cuff also protects processed transcripts from degradation by the exonuclease Rat1. Our work reveals a conceptually different mechanism of transcriptional enhancement. In contrast to other factors that regulate termination by binding to specific signals on nascent RNA, the RDC complex inhibits termination in a chromatin-dependent and sequence-independent manner.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROMOTES TRANSCRIPTION TERMINATION; DROSOPHILA-MELANOGASTER; CLUSTERS; GERMLINE; COMPLEX; MECHANISM; DEFENSE; PATHWAY; PROTEIN; GENOME; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Apr 2019 11:56 |
| Last Modified: | 02 Apr 2019 11:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3639 |
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