Zhou, Rong and Treeck, Oliver and Horn, Felicitas and Ortmann, Olaf (2005) Effects of prolonged tamoxifen treatment on receptor expression and apoptosis of ovarian cancer cells. GYNECOLOGIC ONCOLOGY, 96 (3). pp. 678-683. ISSN 0090-8258, 1095-6859
Full text not available from this repository. (Request a copy)Abstract
Objective. Tamoxifen, which is widely used in the treatment of breast cancer, also has a beneficial effect on cisplatin-refractory ovarian cancer, In this study, we investigated the long-term effects of this drug on estrogen-receptor-positive ovarian cancer cells. Methods. We performed an in vitro selection process by long-term treatment of BG-1 ovarian cancer cells with 4-hydroxy tamoxifen (4-OH TAM). Drug effects on cell growth were determined by measurement of relative cell numbers (MTS assay), the apoptotic effects of 4-OH TAM were determined by analysis of poly (ADP-ribose) polymerase (PARP) cleavage and by ELISA measurement of DNA-histone complexes in cytoplasm. Results. Analysis of BG-1(LT) ovarian cancer cells isolated after 5 months of long-term treatment with 4-OH TAM revealed both a significantly reduced apoptotic and antiproliferative effect of this drug. Further experiments to examine expression changes of the receptor tyrosine kinases EGFR, HER2 and estrogen receptor a did not reveal any alterations in BG-1 (LT) if compared to wild-type cells. In contrast, in this cell line, a significant alteration in the expression of estrogen receptor beta was observed. Conclusion. Our findings indicate that long-term treatment with 4-OH TAM is able to diminish both the antiproliferative and apoptotic action of this drug on BG-1 ovarian cancer cells. Our data suggest that the responsiveness of ovarian cancer cells to 4-OH TAM decreases after long-term treatment with this drug in vitro like previously observed after long-term treatment of breast cancer cells. (c) 2004 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR RECEPTOR; HUMAN BREAST-CANCER; ESTROGEN-RECEPTOR; IN-VITRO; CARCINOMA; LINES; PROLIFERATION; RESISTANCE; PROTEIN; SENSITIVITY; estrogen receptor modulator; tamoxifen; proliferation; apoptosis; drug resistance; ovarian cancer cell line |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 May 2021 05:49 |
| Last Modified: | 21 May 2021 05:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36415 |
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