Buettner, R and Straub, RH and Ottinger, I and Woenckhaus, M and Scholmerich, J and Bollheimer, LC (2005) Efficient analysis of hepatic glucose output and insulin action using a liver slice culture system. HORMONE AND METABOLIC RESEARCH, 37 (3). pp. 127-132. ISSN 0018-5043,
Full text not available from this repository. (Request a copy)Abstract
Background: Liver slices have been reported to retain histological integrity and metabolic capacity for over 24 hours in flask culture systems, and they have been used for pharmacological and toxicological studies before. However, whether this method is suitable to measure hepatic glucose output is unknown. Methods: Precision-cut liver slices were prepared from fresh male rat liver. After high-glucose pre-incubation (11.2 mmol/l), medium was changed to low-glucose conditions (0.5 mmol/l). Glucose and lactate levels as well as aspartate aminotransferase activity were monitored for 50 minutes with or without addition of insulin (600 mu mol/l) and/or epinephrine (0.5 mu mol/l). Slice potassium content and histology were examined to prove liver viability. Results: We observed a stable glucose production from the liver slices of 0.3-0.4 mu mol/g liver/min. Epinephrine increased (by 82 +/- 30%) and insulin decreased (by 80 +/- 8%) liver slice glucose output. Significant signs of ischemia were not detected. Conclusions: Hepatic glucose release can be reliably measured in a liver slice culture system, and it is regulated by major hormone systems. This method may be helpful for further characterization of direct insulin action and resistance in a complex tissue as the liver; however, pharmacological applications such as the analysis of drug effects on hepatic glucose metabolism can also be envisioned.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRIMARY MONOLAYER-CULTURE; RAT-LIVER; TISSUE-SLICES; FAT; HEPATOCYTES; METABOLISM; MECHANISMS; MODEL; BASAL; hepatic glucose output; liver slice culture; insulin; epinephrine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 May 2021 14:08 |
| Last Modified: | 20 May 2021 14:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36417 |
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