Morphological and molecular investigations of Tubulinosema ratisbonensis gen. nov., sp nov (Microsporidia : Tubulinosematidae fam. nov.), a parasite infecting a laboratory colony of Drosophila melanogaster (Diptera : Drosophilidae)

Franzen, C and Fischer, S and Schroeder, J and Scholmerich, J and Schneuwly, S (2005) Morphological and molecular investigations of Tubulinosema ratisbonensis gen. nov., sp nov (Microsporidia : Tubulinosematidae fam. nov.), a parasite infecting a laboratory colony of Drosophila melanogaster (Diptera : Drosophilidae). JOURNAL OF EUKARYOTIC MICROBIOLOGY, 52 (2). pp. 141-152. ISSN 1066-5234, 1550-7408

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Abstract

Anew species of microsporidia from Drosophila melanogaster was investigated by light and electron microscopy and by ribosomal RNA (rRNA) sequencing. This microsporidium and the previously described Nosema kingi and Nosema acridophagus have been transferred to the new genus Tubulinosema gen. nov. with the following characters: nuclei are in diplokaryotic arrangement during the life cycle. All stages are in direct contact with the host cell cytoplasm, slightly anisofilar polar tube with the last coils being smaller in diameter arranged in one or two rows on both sides of the diplokaryon and small tubuli on the surface of late meronts. Spores are oval or slightly pyriform. Thick endospore wall, thinner over anchoring disc. This new genus and the genus Brachiola have been placed in a new family Tubulinosematidae fam. nov. Phylogenetic analysis of small subunit rRNA sequences by different methods placed Tubulinosema spp. in one clade with the genus Brachiola forming its sister clade, which is distant from the clade containing the true Nosema spp. including Nosema bombycis.

Item Type: Article
Uncontrolled Keywords: RIBOSOMAL-RNA; WILLISTONI STURTEVANT; NOSEMA-KINGI; SP N; ULTRASTRUCTURE; PHYLOGENY; Nosema; parasite; rRNA; taxonomy
Subjects: 600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Innere Medizin I
Medicine > Lehrstuhl für Pathologie
Biology, Preclinical Medicine > Institut für Zoologie > Entwicklungsbiologie (Prof. Dr. Stephan Schneuwly)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 20 May 2021 14:58
Last Modified: 20 May 2021 14:58
URI: https://pred.uni-regensburg.de/id/eprint/36427

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