Wuelbeck, Corinna and Szabo, Gisela and Shafer, Orie T. and Helfrich-Foerster, Charlotte and Stanewsky, Ralf (2005) The novel Drosophila tim(blind) mutation affects behavioral rhythms but not periodic eclosion. GENETICS, 169 (2). pp. 751-766. ISSN 0016-6731, 1943-2631
Full text not available from this repository. (Request a copy)Abstract
Circadian clock function depends on the tightly regulated exclusion or presence of clock proteins within the nucleus. A newly induced long-period timeless mutant, tim(blind), encodes a constitutively hypophosphorylated TIM protein. The mutant protein is not properly degraded by light, and tim(blind) flies show abnormal behavioral responses to light pulses. This is probably caused by impaired nuclear accumulation of TIMBLIND protein, which we observed in brain pacemaker neurons and photoreceptor cells of the compound eye. tim(blind) encodes two closely spaced amino acid changes compared to the wild-type TIM protein; one of them is within a putative nuclear export signal of TIM. Under constant conditions, tim(blind) flies exhibit 26hr free-running locomotor rhythms, which are not correlated with a period lengthening of eclosion rhythms and period-luciferase reporter-gene oscillations. Therefore it seems possible that TIM-in addition to its well-established role as core clock factor-functions as a clock output factor, involved in determining the period length of adult locomotor rhythms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CLOCK PROTEINS PERIOD; PHASE RESPONSE CURVES; CIRCADIAN-CLOCK; GENE-EXPRESSION; PACEMAKER NEURONS; TIMELESS PROTEIN; NERVOUS-SYSTEM; VISUAL-SYSTEM; MELANOGASTER; LIGHT; |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Alumni or Retired > Organisation der inneren Uhr auf neuronaler Ebene (Prof. Dr. Charlotte Förster) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 May 2021 05:38 |
| Last Modified: | 31 May 2021 05:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36518 |
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